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- Article] Autofluorescence spectroscopy in whole organs with a mobile detector system
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DocNo of ILP: 5480
Doc. Type: Article
Title: Autofluorescence spectroscopy in whole organs with a mobile detector system
Authors: Hansch, A; Sauner, D; Hilger, I; Bottcher, J; Malich, A; Frey, O; Brauer, R; Kaiser, WA
Full Name of Authors: Hansch, A; Sauner, D; Hilger, I; Bottcher, J; Malich, A; Frey, O; Brauer, R; Kaiser, WA
Keywords by Author: autofluorescence; excitation; emission matrices; NAD(P)H; collagen/elastin
Keywords Plus: LASER-INDUCED FLUORESCENCE; ADVANCED GLYCATION; PROTOPORPHYRIN-IX; END-PRODUCTS; REDUCTION; TISSUE; CELLS; MITOCHONDRIA; NEOPLASIA; COLLAGEN
Abstract: Rationale and Objectives. Autofluorescence can be exploited to obtain spectroscopic information about tissues or organs in a noninvasive fashion. The knowledge of normal organ patterns is a prerequisite for subsequent characterization of pathological states, eg, inflammation or tumors. Therefore, the aim of this study was to investigate the autofluorescence properties of healthy organs in mice. Materials and Methods. Organs from C57B1/6 mice were removed in toto and stored in physiologic sodium chloride solution on ice (non-perfused specimens). Investigations were performed with a custom-made mobile fluorescence detector. Excitation-emission matrices (EEMs) were measured in selected organs (bladder, brain, kidney, liver, and spleen) (n = 5). Afterwards, single-emission spectra were obtained in selected organs (bladder, colon, brain, kidney, liver, and spleen) and peak fluorescence signal intensities were calculated (n = 9). Results. EEMs showed that excitation at wavelengths from 300-310 nm (emission spectra in all samples of bladder and brain; probably caused by collagen/elastin) and from 350-360 nm (emission spectra in all samples with the exception of spleen; probably caused by NAD(P)H) seem to be best suited for autofluorescence measurements in organs. The single-emission spectra measurements were noticeably different in terms of occurrence (yes/no response) and intensity of fluorescence emission peaks in different organs. Conclusion. Combined autofluorescence measurements of collagen/elastin (for structural information) and NAD(P)H (for functional information) allow conclusions about the target organs. Therefore, autofluorescence measurements seem to be a diagnostic tool feasible for characterization of tissue.
Cate of OECD: Clinical medicine
Year of Publication: 2004
Business Area: other
Detail Business: medicine & science
Country: USA
Study Area:
Name of Journal: ACADEMIC RADIOLOGY
Language: English
Country of Authors: Univ Jena, Inst Diagnost & Intervent Radiol, D-07743 Jena, Germany; Univ Jena, Inst Pathol, D-6900 Jena, Germany
Press Adress: Hansch, A (reprint author), Univ Jena, Inst Diagnost & Intervent Radiol, Bachstr 18, D-07743 Jena, Germany.
Email Address: andreas.hansch@med.unijena.de
Citaion:
Funding:
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Number of Citaion: 33
Publication: ASSOC UNIV RADIOLOGISTS
City of Publication: OAK BROOK
Address of Publication: 820 JORIE BLVD, OAK BROOK, IL 60523-2251 USA
ISSN: 1076-6332
29-Character Source Abbreviation: ACAD RADIOL
ISO Source Abbreviation: Acad. Radiol.
Volume: 11
Version: 11
Start of File: 1229
End of File: 1236
DOI: 10.1016/j.acra.2004.07.027
Number of Pages: 8
Web of Science Category: Radiology, Nuclear Medicine & Medical Imaging
Subject Category: Radiology, Nuclear Medicine & Medical Imaging
Document Delivery Number: 874FZ
Unique Article Identifier: WOS:000225337600005
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