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- Article] Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy
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DocNo of ILP: 2545
Doc. Type: Article
Title: Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy
Authors: Olivier, N; Luengo-Oroz, MA; Duloquin, L; Faure, E; Savy, T; Veilleux, I; Solinas, X; Debarre, D; Bourgine, P; Santos, A; Peyrieras, N; Beaurepaire, E
Full Name of Authors: Olivier, Nicolas; Luengo-Oroz, Miguel A.; Duloquin, Louise; Faure, Emmanuel; Savy, Thierry; Veilleux, Israel; Solinas, Xavier; Debarre, Delphine; Bourgine, Paul; Santos, Andres; Peyrieras, Nadine; Beaurepaire, Emmanuel
Keywords by Author:
Keywords Plus: 3RD-HARMONIC GENERATION MICROSCOPY; 2ND-HARMONIC GENERATION; DEVELOPMENTAL BIOLOGY; HARMONIC-GENERATION; ZYGOTIC TRANSITION; ORIGIN; EMBRYOGENESIS; FLUORESCENCE
Abstract: Quantifying cell behaviors in animal early embryogenesis remains a challenging issue requiring in toto imaging and automated image analysis. We designed a framework for imaging and reconstructing unstained whole zebrafish embryos for their first 10 cell division cycles and report measurements along the cell lineage with micrometer spatial resolution and minute temporal accuracy. Point-scanning multiphoton excitation optimized to preferentially probe the innermost regions of the embryo provided intrinsic signals highlighting all mitotic spindles and cell boundaries. Automated image analysis revealed the phenomenology of cell proliferation. Blastomeres continuously drift out of synchrony. After the 32-cell stage, the cell cycle lengthens according to cell radial position, leading to apparent division waves. Progressive amplification of this process is the rule, contrasting with classical descriptions of abrupt changes in the system dynamics.
Cate of OECD: Other natural sciences
Year of Publication: 2010
Business Area: other
Detail Business: medicine & science
Country: USA
Study Area:
Name of Journal: SCIENCE
Language: English
Country of Authors: [Duloquin, Louise; Peyrieras, Nadine] CNRS, Inst Neurobiol Alfred Fessard, Gif Sur Yvette, France; [Olivier, Nicolas; Veilleux, Israel; Solinas, Xavier; Debarre, Delphine; Beaurepaire, Emmanuel] Ecole Polytech, CNRS, INSERM, Lab Opt & Biosci, F-91128 Palaiseau, France; [Luengo-Oroz, Miguel A.; Santos, Andres] Univ Politecn Madrid, Madrid, Spain; [Luengo-Oroz, Miguel A.; Santos, Andres] CIBER, Biomed Res Ctr, BBN, Madrid, Spain; [Faure, Emmanuel; Savy, Thierry; Bourgine, Paul] Ecole Polytech, CNRS, Ctr Rech Epistemol Appl, F-75230 Paris, France; [Bourgine, Paul] Reseau Natl Syst Complexes, Paris, France; [Peyrieras, Nadine] Inst Syst Complexes Paris Ile de France, Paris, France
Press Adress: Peyrieras, N (reprint author), CNRS, Inst Neurobiol Alfred Fessard, Gif Sur Yvette, France.
Email Address: nadine.peyrieras@inaf.cnrs-gif.fr; emmanuel.beaurepaire@polytechnique.edu
Citaion: Santos, Andres/0000-0001-7423-9135; Beaurepaire, Emmanuel/0000-0002-2082-8214
Funding: Direction Generale de l'Armament; Agence Nationale de la Recherche,; FP6 New Emerging Science and Technology program; Association pour le Recherche Contre le Cancer; Egide Picasso France-Spain; Fondation Louis D.; Institut de France
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Number of Citaion: 27
Publication: AMER ASSOC ADVANCEMENT SCIENCE
City of Publication: WASHINGTON
Address of Publication: 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA
ISSN: 0036-8075
29-Character Source Abbreviation: SCIENCE
ISO Source Abbreviation: Science
Volume: 329
Version: 5994
Start of File: 967
End of File: 971
DOI: 10.1126/science.1189428
Number of Pages: 5
Web of Science Category: Multidisciplinary Sciences
Subject Category: Science & Technology - Other Topics
Document Delivery Number: 640WN
Unique Article Identifier: WOS:000281084800041
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