경마 | Cases & Studies in Horse Racing | 赛马
- date : 2015-05-20 01:10|hit : 1903
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- Article] MSTN genotypes in Thoroughbred horses influence skeletal muscle gene expression and racetrack performance
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DocNo of ILP: 1020
Doc. Type: Article
Title: MSTN genotypes in Thoroughbred horses influence skeletal muscle gene expression and racetrack performance
Authors: McGivney, BA; Browne, JA; Fonseca, RG; Katz, LM; MacHugh, DE; Whiston, R; Hill, EW
Full Name of Authors: McGivney, Beatrice A.; Browne, John A.; Fonseca, Rita G.; Katz, Lisa M.; MacHugh, David E.; Whiston, Ronan; Hill, Emmeline W.
Keywords by Author: equine; exercise; gene expression; myostatin; skeletal muscle; training
Keywords Plus: ASSOCIATION; SELECTION
Abstract: Myostatin, encoded by the MSTN gene, is a member of the TGF-beta superfamily that regulates skeletal muscle development. A MSTN SNP significantly associated with Thoroughbred horse racing phenotypes has recently been identified as well as significant reductions in Thoroughbred skeletal muscle gene expression for three transcripts 4001500 base pairs downstream of the MSTN gene following a period of training. Together, these findings indicate that MSTN genotypes may influence MSTN gene expression. To investigate this, MSTN mRNA expression was measured in biopsies from the middle gluteal muscle from 60 untrained yearling Thoroughbreds (C/C, n = 15; C/T, n = 28; T/T, n = 17) using two independent real-time qRT-PCR assays. MSTN gene expression was also evaluated in a subset (N = 33) of these animals using samples collected after a ten-month period of training. A significant association was observed between genotype and mRNA abundance for the untrained horses (assay I, P = 0.0237; assay II, P = 0.003559), with the C/C cohort having the highest MSTN mRNA levels, the T/T group the lowest levels and the C/T group intermediate levels. Following training, there was a significant decrease in MSTN mRNA (-3.35-fold; P = 6.9 x 10-7), which was most apparent for the C/C cohort (-5.88-fold, P = 0.001). These data demonstrate the tight relationship between phenotype, genotype and gene expression at the MSTN gene in Thoroughbred racehorses.
Cate of OECD: Animal and dairy science
Year of Publication: 2012
Business Area: horse race
Detail Business: horse race
Country: USA
Study Area:
Name of Journal: ANIMAL GENETICS
Language: English
Country of Authors: [McGivney, Beatrice A.; Browne, John A.; MacHugh, David E.; Whiston, Ronan; Hill, Emmeline W.] Univ Coll Dublin, Coll Agr Food Sci & Vet Med, UCD Sch Agr & Food Sci, Anim Genom Lab, Dublin 4, Ireland; [Fonseca, Rita G.; Katz, Lisa M.] Univ Coll Dublin, Coll Agr Food Sci & Vet Med, UCD Sch Vet Med, Univ Vet Hosp, Dublin 4, Ireland; [MacHugh, David E.] Univ Coll Dublin, UCD Conway Inst Biomol & Biomed Res, Dublin 4, Ireland
Press Adress: Hill, EW (reprint author), Univ Coll Dublin, Coll Agr Food Sci & Vet Med, UCD Sch Agr & Food Sci, Anim Genom Lab, Dublin 4, Ireland.
Email Address: Emmeline.Hill@ucd.ie
Citaion:
Funding:
Lists of Citation: Amthor H, 2007, P NATL ACAD SCI USA, V104, P1835, DOI 10.1073/pnas.0604893104; Binns MM, 2010, ANIM GENET, V41, P154, DOI 10.1111/j.1365-2052.2010.02126.x; Gu JJ, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0005767; GUNN HM, 1978, RES VET SCI, V24, P277; Hennebry A, 2009, AM J PHYSIOL-CELL PH, V296, pC525, DOI 10.1152/ajpcell.00259.2007; Hill EW, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0008645; Hill EW, 2010, ANIM GENET, V41, P56, DOI 10.1111/j.1365-2052.2010.02104.x; McGivney BA, 2010, BMC GENOMICS, V11, DOI 10.1186/1471-2164-11-398; McPherron AC, 1997, NATURE, V387, P83, DOI 10.1038/387083a0; Mosher DS, 2007, PLOS GENET, V3, P779, DOI 10.1371/journal.pgen.0030079; Purcell S, 2007, AM J HUM GENET, V81, P559, DOI 10.1086/519795; Schuelke M, 2004, NEW ENGL J MED, V350, P2682, DOI 10.1056/NEJMoa040933; Tozaki T, 2010, ANIM GENET, V41, P28, DOI 10.1111/j.1365-2052.2010.02095.x
Number of Citaion: 13
Publication: WILEY-BLACKWELL
City of Publication: HOBOKEN
Address of Publication: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
ISSN: 0268-9146
29-Character Source Abbreviation: ANIM GENET
ISO Source Abbreviation: Anim. Genet.
Volume: 43
Version: 6
Start of File: 810
End of File: 812
DOI: 10.1111/j.1365-2052.2012.02329.x
Number of Pages: 3
Web of Science Category: Agriculture, Dairy & Animal Science; Genetics & Heredity
Subject Category: Agriculture; Genetics & Heredity
Document Delivery Number: 023VF
Unique Article Identifier: WOS:000310064000023
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