학제간연구 | Interdisciplinary Studies in Gambling | 跨学科研究
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- Article] Molecular Characterization and Expression Analysis of Equine Vascular Endothelial Growth Factor Alpha (VEGF alpha) Gene in Horse (Equus caballus)
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DocNo of ILP: 21
Doc. Type: Article
Title: Molecular Characterization and expression Analysis of Equine Vascular Endothelial Growth Factor Alpha (VEGF alpha) Gene in Horse (Equus caballus)
Authors: Song, KD; Cho, HW; Lee, HK; Cho, BW
Full Name of Authors: Song, Ki-Duk; Cho, Hyun-Woo; Lee, Hak-Kyo; Cho, Byung Wook
Keywords by Author: Differentially Expressed Gene; Exercise; Horse; Quantitative-Polymerase Chain Reaction; Vascular Endothelial Growth Factor
Keywords Plus: MESSENGER-RNA RESPONSES; STANDARD-BRED TROTTERS; SKELETAL-MUSCLE; SYNOVIAL-FLUID; EXERCISE
Abstract: The objective of this study was to determine the molecular characteristics of the horse vascular endothelial growth factor alpha gene (VEGF alpha) by constructing a phylogenetic tree, and to investigate gene expression profiles in tissues and blood leukocytes after exercise for development of suitable biomarkers. Using published amino acid sequences of other vertebrate species (human, chimpanzee, mouse, rat, cow, pig, chicken and dog), we constructed a phylogenetic tree which showed that equine VEGF alpha belonged to the same clade of the pig VEGF alpha. Analysis for synonymous (Ks) and non-synonymous substitution ratios (Ka) revealed that the horse VEGF alpha underwent positive selection. RNA was extracted from blood samples before and after exercise and different tissue samples of three horses. expression analyses using reverse transcription-polymerase chain reaction (RT-PCR) and quantitative-polymerase chain reaction (qPCR) showed ubiquitous expression of VEGF alpha mRNA in skeletal muscle, kidney, thyroid, lung, appendix, colon, spinal cord, and heart tissues. Analysis of differential expression of VEGF alpha gene in blood leukocytes after exercise indicated a unimodal pattern. These results will be useful in developing biomarkers that can predict the recovery capacity of racing horses.
Cate of OECD: Animal and dairy science
Year of Publication: 2014
Business Area: horse race
Detail Business: racehorse
Country: South Korea
Study Area:
Name of Journal: ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
Language: English
Country of Authors: [Song, Ki-Duk; Lee, Hak-Kyo] Hankyong Natl Univ, Genom Informat Ctr, Anseong 456649, South Korea; [Cho, Hyun-Woo; Cho, Byung Wook] Pusan Natl Univ, Coll Nat Resources & Life Sci, Dept Anim Sci, Miryang 627706, South Korea
Press Adress: Cho, BW (reprint author), Pusan Natl Univ, Coll Nat Resources & Life Sci, Dept Anim Sci, Miryang 627706, South Korea.
Email Address: breedlee@empas.com; bwcho@pusan.ac.kr
Citaion:
Funding: Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ008106, PJ008196]
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Number of Citaion: 24
Publication: ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC
City of Publication: SEOUL
Address of Publication: ROOM 708 SAMMO SPOREX, 1638-32, SEOWON-DONG, GWANAK-GU, SEOUL 151-730, SOUTH KOREA
ISSN: 1011-2367
29-Character Source Abbreviation: ASIAN AUSTRAL J ANIM
ISO Source Abbreviation: Asian Australas. J. Anim. Sci.
Volume: 27
Version: 5
Start of File: 743
End of File: 748
DOI: 10.5713/ajas.2013.13821
Number of Pages: 6
Web of Science Category: Agriculture, Dairy & Animal Science
Subject Category: Agriculture
Document Delivery Number: AG7TL
Unique Article Identifier: WOS:000335621600016
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