학제간연구 | Interdisciplinary Studies in Gambling | 跨学科研究
- date : 2015-05-20 01:10|hit : 1457
-
- Article] Identification and quantification of metabolites common to 17 alpha-methyltestosterone and mestanolone in horse urine
-
DocNo of ILP: 4124
Doc. Type: Article
Title: Identification and quantification of metabolites common to 17 alpha-methyltestosterone and mestanolone in horse urine
Authors: Yamada, M; Aramaki, S; Okayasu, T; Hosoe, T; Kurosawa, M; Kijima-Suda, I; Saito, K; Nakazawa, H
Full Name of Authors: Yamada, Masayuki; Aramaki, Sugako; Okayasu, Toshimasa; Hosoe, Tomoo; Kurosawa, Masahiko; Kijima-Suda, Isao; Saito, Koichi; Nakazawa, Hiroyuki
Keywords by Author: 17 alpha-methyltestosterone; mestanolone; equine; metabolite; anabolic-androgenic steroid; GC/MS
Keywords Plus: ANABOLIC-STEROIDS; BIOTRANSFORMATION; SPECTROMETRY; EQUINE; HUMANS
Abstract: Anabolic steroids with the 17 alpha-methyl,17 beta-hydroxyl group, which were developed as oral formulations for therapeutic purposes, have been abused in the field of human sports. These anabolic steroids are also used to enhance racing performance in racehorses. In humans, structurally related 17 alpha-methyltestosterone (MTS) and mestanolone (MSL), which are anabolic steroids with the 17 alpha-methyl, 17 beta-hydroxyl group, have metabolites in common. The purpose of this study was to determine metabolites common to these two steroids in horses, which may serve as readily available screening targets for the doping test of these steroids in racehorses. Urine sample collected after administering MTS and MSL to horses was treated to obtain unconjugated steroid, glucuronide, and sulfate fractions. The fractions were subjected to gas chromatography/mass spectrometry (GC/MS), and 17 alpha-methyl-5 alpha-androstan-3 beta,17 beta-diol, 17 alpha-hydroxymethyl-5 alpha-androstan-3 beta,17 beta-diol, 17 alpha-methyl-5 alpha-androstan-3 beta,16 beta,17 beta-triol, and 17 alpha-methyl-5 alpha-androstan-3 beta, 16 alpha, 17 beta-triol were detected as the common metabolites by comparison with synthesized reference standards. The urinary concentrations of these metabolites after dosing were determined by GOMS. 17 alpha-Methyl-5 alpha-androstan-3 beta,16 beta,17 beta-triol was mainly detected in the sulfate fractions of urine samples after administration. This compound was consistently detected for the longest time in the urine samples after dosing with both steroids. The results suggest that 17 alpha-methyl-5 alpha-androstan-3 beta, 16 beta, 17 beta-triol is a very useful screening target for the doping test of NITS and MSL in racehorses. (C) 2007 Elsevier B.V. All rights reserved.
Cate of OECD: Chemical sciences
Year of Publication: 2007
Business Area: horse race
Detail Business: racehorse
Country: Netherlands
Study Area:
Name of Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Language: English
Country of Authors: Lab Racing Chem, Utsunomiya, Tochigi 3200851, Japan; Hoshi Univ, Fac Pharmaceut Sci, Shinagawa Ku, Tokyo 1428501, Japan
Press Adress: Yamada, M (reprint author), Lab Racing Chem, 1731-2 Tsuruta Machi, Utsunomiya, Tochigi 3200851, Japan.
Email Address: m-yamada@lrc.or.jp
Citaion:
Funding:
Lists of Citation: DEHENNIN L, 1980, BIOMED MASS SPECTROM, V7, P493, DOI 10.1002/bms.1200071108; Dumasia MC, 2003, RAPID COMMUN MASS SP, V17, P320, DOI 10.1002/rcm.909; MASSE R, 1991, J CHROMATOGR-BIOMED, V562, P323, DOI 10.1016/0378-4347(91)80588-4; McKinney AR, 2007, ANAL CHIM ACTA, V581, P377, DOI 10.1016/j.aca.2006.08.025; Schanzer W, 1996, CLIN CHEM, V42, P1001; SCHANZER W, 1993, ANAL CHIM ACTA, V275, P23, DOI 10.1016/0003-2670(93)80274-O; SCHANZER W, 1992, STEROIDS, V57, P537, DOI 10.1016/0039-128X(92)90023-3; Stanley SMR, 1997, J CHROMATOGR B, V690, P55, DOI 10.1016/S0378-4347(96)00404-5; TAKEDA F, 1999, RES REPORT LAB RACIN, P1
Number of Citaion: 9
Publication: ELSEVIER SCIENCE BV
City of Publication: AMSTERDAM
Address of Publication: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN: 0731-7085
29-Character Source Abbreviation: J PHARMACEUT BIOMED
ISO Source Abbreviation: J. Pharm. Biomed. Anal.
Volume: 45
Version: 1
Start of File: 125
End of File: 133
DOI: 10.1016/j.jpba.2007.06.020
Number of Pages: 9
Web of Science Category: Chemistry, Analytical; Pharmacology & Pharmacy
Subject Category: Chemistry; Pharmacology & Pharmacy
Document Delivery Number: 219QT
Unique Article Identifier: WOS:000250100400014
- reply : 0
-
- list
-
- prev
- next