학제간연구 | Interdisciplinary Studies in Gambling | 跨学科研究
- date : 2015-05-20 01:10|hit : 1470
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- Article] A simplified method of determining synovial fluid chondroitin sulfate chain length
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DocNo of ILP: 4054
Doc. Type: Article
Title: A simplified method of determining synovial fluid chondroitin sulfate chain length
Authors: Brown, MP; Trumble, TN; Sandy, JD; Merritt, KA
Full Name of Authors: Brown, M. P.; Trumble, T. N.; Sandy, J. D.; Merritt, K. A.
Keywords by Author: chondroitin sulfate chain length; dimethylmethylene blue; fluorophore-assisted carbohydrate electrophoresis; synovial fluid; biomarkers; joint injury; horse
Keywords Plus: ARTICULAR-CARTILAGE; GLYCOSAMINOGLYCANS
Abstract: Objective: To determine whether dimethylmethylene blue (DMMB) analysis, when combined with agarose gel filtration chromatography (Superose 6), can be performed instead of fluorophore-assisted carbohydrate electrophoresis (FACE) to determine chondroitin sulfate (CS) chain length in synovial fluid (SF). Methods: SF was obtained from (1) normal horses after 8 weeks of rest, (2) the same horses after 9 months of treadmill training, and (3) horses with osteochondral (OC) injury from racing. SF CS concentrations and chain lengths were determined by gel chromatography and DMMB analysis and compared with previous results determined by FACE analysis on the same samples. Results: DMMB analysis showed that SF CS peak chain length in the OC injury group increased significantly (18.7 kDa) when compared to rested and exercised normal horses (15.6 kDa). The assay had a positive predictive value of 71% and a negative predictive value of 75% for discriminating between normal and injured joints. Conclusions: We report a simple and inexpensive DMMB analysis of SF CS chain length, which, when coupled with Superose 6 chromatography, discriminates between normal and post-injury joints. Similar to our previous FACE analysis results [Brown MP, Trumble TN, Plaas AHK, Sandy JD, Romano M, Hernandez J, et-al. Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid. Osteoarthritis Cartilage 2007;15], our DMMB results show an increase in the chain length of the CS in the SF of injured joints. (c) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Cate of OECD: Clinical medicine
Year of Publication: 2007
Business Area: other
Detail Business: medicine & science
Country: England
Study Area: globalization, globalization, methosprediction, method, prediction
Name of Journal: OSTEOARTHRITIS AND CARTILAGE
Language: English
Country of Authors: Univ Florida, Coll Vet Med, Dept Large Anim Clin Sci, Gainesville, FL 32610 USA; Rush Univ, Med Ctr, Dept Biochem, Chicago, IL 60612 USA
Press Adress: Brown, MP (reprint author), Univ Florida, Coll Vet Med, Dept Large Anim Clin Sci, Box 100136, Gainesville, FL 32610 USA.
Email Address: brownmu@vetmed.ufl.edu
Citaion:
Funding:
Lists of Citation: Bayliss MT, 1999, J BIOL CHEM, V274, P15892, DOI 10.1074/jbc.274.22.15892; Belcher C, 1997, ANN RHEUM DIS, V56, P299, DOI 10.1136/ard.56.5.299; BROWN MP, 2007, OSTEOARTHR CARTILAGE, P15; Brown MP, 1998, AM J VET RES, V59, P786; DEUTSCH AJ, 1995, J ORTHOPAED RES, V13, P230, DOI 10.1002/jor.1100130211; FARNDALE RW, 1982, CONNECT TISSUE RES, V9, P247, DOI 10.3109/03008208209160269; LEMAIRE M, 1987, BIOCHEM J, V243, P399; Sharif M, 1996, BRIT J RHEUMATOL, V35, P951; SHINMEI M, 1992, ARTHRITIS RHEUM, V35, P1304; Uesaka Shinji, 2001, Journal of Nippon Medical School, V68, P165, DOI 10.1272/jnms.68.165
Number of Citaion: 10
Publication: W B SAUNDERS CO LTD
City of Publication: LONDON
Address of Publication: 32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND
ISSN: 1063-4584
29-Character Source Abbreviation: OSTEOARTHR CARTILAGE
ISO Source Abbreviation: Osteoarthritis Cartilage
Volume: 15
Version: 12
Start of File: 1443
End of File: 1445
DOI: 10.1016/j.joca.2007.05.018
Number of Pages: 3
Web of Science Category: Orthopedics; Rheumatology
Subject Category: Orthopedics; Rheumatology
Document Delivery Number: 236SD
Unique Article Identifier: WOS:000251322800012
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