학제간연구 | Interdisciplinary Studies in Gambling | 跨学科研究
- date : 2015-05-20 01:10|hit : 1489
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- Review] A strategy for correlative microscopy of large skin samples: towards a holistic view of axillary skin complexity
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DocNo of ILP: 3962
Doc. Type: Review
Title: A strategy for correlative microscopy of large skin samples: towards a holistic view of axillary skin complexity
Authors: Wilke, K; Wick, K; Keil, FJ; Wittern, KP; Wepf, R; Biel, SS
Full Name of Authors: Wilke, Katrin; Wick, Katrin; Keil, Frerich J.; Wittern, Klaus-Peter; Wepf, Roger; Biel, Stefan S.
Keywords by Author: axillary skin; correlative microscopy; multimodal imaging; sweat gland
Keywords Plus: ELECTRON-MICROSCOPY; SWEAT GLANDS; FINE-STRUCTURE; CELLS; LIGHT; ULTRASTRUCTURE; APPARATUS; MEMBRANE; CULTURE; TISSUE
Abstract: Knowledge about the structural elements of skin and its appendices is an essential prerequisite for understanding their complex functions and interactions. The hence necessary morphological description across several orders of scale not only requires the investigation at the light microscopic level but also ultrastructural investigation, ideally on the identical sample. For a correlative and multimodal observation one unique preparation protocol is mandatory. As a compromise between sample sizes of > 500 mu m in diameter on the one hand and optimal preservation of antigenicity and morphology on the other, we developed a new preparation protocol that allows (i) 3D reconstruction of the resin-embedded sample by confocal light microscopy prior to (ii) direct immunolocalization of target proteins within selected sample planes by light and fluorescence microscopy or transmission electron microscopy. Alternatively, (iii) serial cryosections of the frozen sample can be taken for characterizing the sample in toto. With this unique approach we were able to fully demonstrate the structural complexity of axillary skin samples, increasing the structural resolution from 3D reconstruction of the whole gland up to ultrastructural investigations at the subcellular level. We could demonstrate that axillary sweat glands are not separately distributed, as has been assumed to date; instead, they seem to be intricately twisted into one another. This promotes the concept of a complex axillary sweat gland organ instead of single sweat gland entities.
Cate of OECD: Clinical medicine
Year of Publication: 2008
Business Area: other
Detail Business: medicine & science
Country: England
Study Area: correlation, correlation
Name of Journal: EXPERIMENTAL DERMATOLOGY
Language: English
Country of Authors: [Wilke, Katrin; Wick, Katrin; Wittern, Klaus-Peter; Biel, Stefan S.] Beiersdorf AG, D-20245 Hamburg, Germany; [Wilke, Katrin; Keil, Frerich J.] Tech Univ Hamburg, D-2100 Hamburg, Germany; [Wick, Katrin] Fachhochschule Furtwangen, Furtwangen, Germany; [Wepf, Roger] ETH, Elect Microscopy Ctr, Zurich, Switzerland
Press Adress: Biel, SS (reprint author), Beiersdorf AG, PO Box 663,Unnastrasse 48, D-20245 Hamburg, Germany.
Email Address: stefan.biel@beiersdorf.com
Citaion:
Funding:
Lists of Citation: BAKER DJ, 2002, GLANDS APOCRINE GLAN; Biel SS, 2003, J MICROSC-OXFORD, V212, P91, DOI 10.1046/j.1365-2818.2003.01227.x; CHARLES A, 1960, J INVEST DERMATOL, V34, P81; CHARLES A, 1959, J ANAT, V93, P226; DERHAUT PF, 1927, HDB HAUT GESCHLECHTS, V1, P1; Goldsmith LA, 1998, DERMATOLOGY GEN MED, P155; Gorzelanny C, 2006, EXP DERMATOL, V15, P387, DOI 10.1111/j.0906-6705.2006.00424.x; Groscurth P, 2002, CURR PROBL DERMATOL, V30, P1; HASHIMOT.K, 1966, J INVEST DERMATOL, V46, P172; HASHIMOT.K, 1966, J INVEST DERMATOL, V46, P378; Hashimoto K, 1986, BIOL INTEGUMENT, V2, P339; HIBBS RG, 1958, AM J ANAT, V103, P201, DOI 10.1002/aja.1001030204; HIBBS RG, 1962, J INVEST DERMATOL, V38, P77; HOHENBERG H, 1994, J MICROSC-OXFORD, V175, P34; Hohenberg H, 1996, J MICROSC-OXFORD, V183, P133, DOI 10.1046/j.1365-2818.1996.820642.x; Huppertz B, 1998, CELL TISSUE RES, V291, P133; KANEKO Y, 1995, J ELECTRON MICROSC, V44, P104; KATSEN AD, 1992, SCANNING, V14, P225; Mironov AA, 2000, TRENDS CELL BIOL, V10, P349, DOI 10.1016/S0962-8924(00)01787-6; Muller-Reichert T, 2007, METHOD CELL BIOL, V79, P101, DOI 10.1016/S0091-679X(06)79004-5; PFEIFFER S, 2000, J INVEST DERMATOL, V114, P1031; Polishchuk RS, 2000, J CELL BIOL, V148, P45, DOI 10.1083/jcb.148.1.45; Richter T, 2001, APPL PHYS A-MATER, V72, pS125, DOI 10.1007/s003390100750; ROESSLER F, 1991, LARYNGOSCOPE, V101, P473; Sato K, 1987, AM J PHYSIOL, V252, P166; Sawaguchi A, 2002, J MICROSC-OXFORD, V208, P158, DOI 10.1046/j.1365-2818.2002.01085.x; Schiefferdecker P., 1922, Zoologica Stuttgart, V27, P1; Serizawa Y, 2001, HISTOL HISTOPATHOL, V16, P453; STUDER D, 1995, J MICROSC-OXFORD, V179, P321; Tiedemann J, 1998, J MICROSC-OXFORD, V189, P163; WEPF R, 2006, BIOENGINEERING SKIN, P149; WILKE K, 2004, J INVEST DERMATOL, V123, pA93; Wilke K, 2006, SKIN PHARMACOL PHYSI, V19, P38, DOI 10.1159/000089142; 1994, ELECT MICROSC ICEM 1, V3, P233
Number of Citaion: 34
Publication: BLACKWELL PUBLISHING
City of Publication: OXFORD
Address of Publication: 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
ISSN: 0906-6705
29-Character Source Abbreviation: EXP DERMATOL
ISO Source Abbreviation: Exp. Dermatol.
Volume: 17
Version: 1
Start of File: 73
End of File: 80
DOI: 10.1111/j.1600-0625.2007.00635.x
Number of Pages: 8
Web of Science Category: Dermatology
Subject Category: Dermatology
Document Delivery Number: 239XX
Unique Article Identifier: WOS:000251552700011
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