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- Article] Expression of the Escherichia coli NRZ nitrate reductase is highly growth phase dependent and is controlled by RpoS, the alternative vegetative sigma factor
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DocNo of ILP: 7127
Doc. Type: Article
Title: expression of the Escherichia coli NRZ nitrate reductase is highly growth phase dependent and is controlled by RpoS, the alternative vegetative sigma factor
Authors: Chang, L; Wei, LLC; Audia, JP; Morton, RA; Schellhorn, HE
Full Name of Authors: Chang, L; Wei, LLC; Audia, JP; Morton, RA; Schellhorn, HE
Keywords by Author:
Keywords Plus: INTEGRATION HOST FACTOR; NARK GENE-exPRESSION; NARGHJI OPERON; LYSINE DECARBOXYLASE; CODON USAGE; K-12; IDENTIFICATION; SEQUENCE; PROMOTER; FNR
Abstract: In the absence of oxygen, many bacteria preferentially use nitrate as a terminal electron acceptor for anaerobic respiration. In Escherichia coli, there are two membrane-bound, differentially regulated nitrate reductases. While the physiological basis for this metabolic redundancy is not completely understood, during exponential growth, synthesis of NRA is greatly induced by anaerobiosis plus nitrate, whereas NRZ is expressed at a low level that is not influenced by anaerobiosis or nitrate. In the course of identifying genes controlled by the stationary phase regulatory factor RpoS (sigma(s)), we found that the expression of NRZ is induced during entry into stationary phase and highly dependent on this alternative sigma factor. expression studies, using operon fusions and nitrate reductase assays, revealed that the NRZ operon is controlled mainly at the level of transcription and is induced 10-fold at the onset of stationary phase in rich media. Consistent with previous reports of RpoS expression, the RpoS dependency of NRZ in minimal media was very high (several hundredfold). We also observed a fivefold stationary phase induction of NRZ in an rpoS background, indicating that other regulatory factors, besides RpoS, are probably involved in transcriptional control of NRZ. The RpoS dependence of NRZ expression was confirmed by Northern analyses using RNA extracted from wild-type and rpoS(-) strains sampled in exponential and stationary phase. In toto, these data indicate that RpoS-mediated regulation of NRZ may be an important physiological adaptation that allows the cell to use nitrate under stress-associated conditions.
Cate of OECD: Biological sciences
Year of Publication: 1999
Business Area: other
Detail Business: medicine & science
Country: England
Study Area: regulation, control, probability, probability, terminal, terminal
Name of Journal: MOLECULAR MICROBIOLOGY
Language: English
Country of Authors: McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada
Press Adress: Schellhorn, HE (reprint author), McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada.
Email Address:
Citaion:
Funding:
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Number of Citaion: 46
Publication: BLACKWELL SCIENCE LTD
City of Publication: OXFORD
Address of Publication: P O BOX 88, OSNEY MEAD, OXFORD OX2 0NE, OXON, ENGLAND
ISSN: 0950-382X
29-Character Source Abbreviation: MOL MICROBIOL
ISO Source Abbreviation: Mol. Microbiol.
Volume: 34
Version: 4
Start of File: 756
End of File: 766
DOI: 10.1046/j.1365-2958.1999.01637.x
Number of Pages: 11
Web of Science Category: Biochemistry & Molecular Biology; Microbiology
Subject Category: Biochemistry & Molecular Biology; Microbiology
Document Delivery Number: 264QQ
Unique Article Identifier: WOS:000084193300012
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