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- Article] Bone morphogenetic proteins promote neurite outgrowth in retinal ganglion cells
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DocNo of ILP: 5293
Doc. Type: Article
Title: Bone morphogenetic proteins promote neurite outgrowth in retinal ganglion cells
Authors: Kerrison, JB; Lewis, RN; Otteson, D; Zack, DJ
Full Name of Authors: Kerrison, JB; Lewis, RN; Otteson, D; Zack, DJ
Keywords by Author:
Keywords Plus: NEUROTROPHIC FACTOR; exPRESSION PATTERNS; CHOLINERGIC NEURONS; BETA SUPERFAMILY; EYE DEVELOPMENT; CHICK RETINA; IN-VITRO; RECEPTORS; SURVIVAL; BMP4
Abstract: Purpose: The purpose of the present study is to test the ability of members of the transforming growth factor/bone morphogenetic protein family to influence retinal ganglion cell (RGC) survival and neurite outgrowth in primary cell culture using a high throughput analysis. Methods: Primary cell cultures were generated using immunoselection of Thy-1 positive cells from dissociated postnatal rat retina and grown on poly-L-lysine/laminin coated 96 well culture dishes in the presence or absence of members of the transforming growth factor/bone morphogenetic protein family. High throughput analysis was performed following fluorescence staining with Hoechst, Calcein AM, and TOTO-3. Outcomes included overall cell survival, survival of cells with neurite outgrowth, and a variety of parameters of neurite outgrowth. Results: Immunomagnetic selection led to an enrichment of cell cultures for RGCs (79%+/- 6.8%). While no significant effect on overall survival was observed with any of the factors tested, members of the bone morphogenetic protein (BMPs) family (BMP2, BMP13, and GDF8 (growth differentiation factor 8)) and BDNF (brain derived neurotrophic factor) increased the number of surviving RGCs with neurite extension in a dose dependent manner. As a group, BMPs increased the number of neurites, length of neurites, and the number of branch points, while BDNF primarily increased neurite length and branch points. Conclusions: We have developed an efficient system that allows for high throughput analysis of cultures enriched for RGCs. Using this assay system, we found that BMPs promote the survival of outgrowth neurons and neurite development in RGC culture.
Cate of OECD: Biological sciences
Year of Publication: 2005
Business Area: other
Detail Business: medicine & science
Country: USA
Study Area:
Name of Journal: MOLECULAR VISION
Language: English
Country of Authors: Johns Hopkins Univ, Dept Ophthalmol, Wilmer Eye Inst, Sch Med,Guerrieri Ctr Genet Engn & Mol Ophthalmol, Baltimore, MD 21287 USA
Press Adress: Kerrison, JB (reprint author), Johns Hopkins Univ, Dept Ophthalmol, Wilmer Eye Inst, Sch Med,Guerrieri Ctr Genet Engn & Mol Ophthalmol, 600 N Wolfe St,Maumenee 813, Baltimore, MD 21287 USA.
Email Address: jkerris1@jhmi.edu
Citaion:
Funding:
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Number of Citaion: 37
Publication: MOLECULAR VISION
City of Publication: ATLANTA
Address of Publication: C/O JEFF BOATRIGHT, LAB B, 5500 EMORY EYE CENTER, 1327 CLIFTON RD, N E, ATLANTA, GA 30322 USA
ISSN: 1090-0535
29-Character Source Abbreviation: MOL VIS
ISO Source Abbreviation: Mol. Vis.
Volume: 11
Version: 24-25
Start of File: 208
End of File: 215
DOI:
Number of Pages: 8
Web of Science Category: Biochemistry & Molecular Biology; Ophthalmology
Subject Category: Biochemistry & Molecular Biology; Ophthalmology
Document Delivery Number: 909DX
Unique Article Identifier: WOS:000227840900001
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